Thermo Fisher Scientific
Pierce™ BCA Protein Assay Kit
23225
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I work on understanding the molecular basis of hedgehog signal transduction at primary cilia. For this purpose, I manipulate the pathway at different levels by knocking out pathway components and testing their effect on the output of the pathway. I also study protein-protein interactions. All of the above mentioned experiments require total protein quantification in the early steps. My cell lysis buffer contains harsh detergents and so BCA assay is suitable for this purpose to accurately quantify protein concentration.
Quantification of proteins
Purified BSA for standard curve determination
Add different amounts of BSA to BCA reagent and incubate at room temperature for 30 min
None
Spectrophotometer
BCA kit from Thermo Fisher is suitable for quantifying proteins in cell extracts after using harsh cell lysis buffers like RIPA buffer.
Very easy to use
Use it for cell lysates prepared in RIPA lysis buffer