Review for APC CD195 (CCR5) Antibody

Cell and Molecular Biology
Northwestern University
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

APC Mouse Anti-Human CD195 Clone 3A9 (RUO)

Catalog Number:

560748

The overal goal of this research is to understand HIV transmission in more details. The specific goal of this experiment was to study the resident target cells of HIV and to see if there are changes in CCR5 expression upon infection.This specific clone was chosen as it is the only clone listed to work in Rhesus Macaque cells. The APC color was chosen cause it fit our flow panel well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Rhesus Macaque PBMCs

Primary Incubation

30 minutes on ice protected from light

Blocking Agent

Staining buffer with FBS from BD

Secondary Incubation

None, anitbody was directly conjugated

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

While our infection was successful we did not see any effect on CCR5 expression.

Additional Notes

None

Related Categories

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Summary

The Good

Ease of use, good signal, good price

The Bad

This antibody is not available in lots of color

The Bottom Line

Works great for flow cytometry applications and this clone is suitable for work with Rhesus Macaque cells.

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