An Efficient Reverse Transcription Kit For Realtime Gene Quantification

Osmania University
Department of Biochemistry
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Thermo Scientific

Product Name:

Verso cDNA synthesis kit

Catalog Number:

AB-1453/B

Verso reverse transcriptase is high efficient thermal stable reverse transcriptase, which can work at wide range of the temperatures. This kit is provided with RT enhancer which gives stability to the RNA. This kit possesses high sensitivity up to 10 picograms of RNA. This kit is provided with individual oligo dT and random hexamers.

Experimental Design and Results Summary

Application

Cloning/ Quantitative real time PCR

Starting Material

Total RNA

Protocol Overview

4 microliters of 5x buffer taken for each individual reaction with 1 microgram of RNA, dNTPs, RNA enhancer, and enzyme as described by protocol and made up to 20 microliters final volume and incubated at 42 degrees for 30 minutes.

Tips

Do not take more than 1 microgram of RNA, which interfere with the reverse transcription process.

Results Summary

Good quality cDNA is obtained after 30 minutes reaction process

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Single step reaction process, which reduces the reaction process to 30 minutes

The Bad

Costly

The Bottom Line

I would recommend this kit for fast hassle free cDNA synthases for your real time PCR process.

Join the discussion