Abcam
Anti-DNAJC3
ab72885
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Browse Similar Products: DNAJC3 Western Blot Antibodies
This protein is involved in the unfolded protein response and may inhibit eIF2alpha phosphorylation and therefore PERK activity. Determining the amount if p58 present due to a variety of stress conditions may be helpful in assessing the severity of ER stress
Western Blot
H4IIE rat hepatoma liver cells
1:1000 in 1:3 odyssey block:1X TBST (0.1% Tween) shaking, overnight at 4 degrees C
1:3 odyssey block:1X TBS at room temperature for 1 hr.
1:15000 Goat anti-rabbit IRDye in 1:3 odyssey block:1X TBST (0.1% Tween) and 0.2% SDS for one hour at room temperature in the dark
N/A
Licor Odyssey
This antibody was predicted to show up at 58kDa. Nothing was there but two huge bands showed up around 30kDa.
None
Nothing.
Everything. Didn't show up in this cell line.
Don't use this with H4IIE liver cells.