Santa Cruz Biotechnology
PERK antibody (H-300)
sc-13073
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Browse Similar Products: PERK Western Blot Antibodies
PERK is a key protein that is phosphorylated and activated during ER stress, responsible for the phosphorylation eIF2alpha. Determining the activation state of PERK due to a variety of stress conditions is helpful in assessing the severity of ER stress.
Western Blot
H4IIE rat hepatoma liver cells
1:500 in 1:3 odyssey block:1X TBST (0.1% Tween) shaking, overnight at 4 degrees C
1:3 odyssey block:1X TBS at room temperature for 1 hr.
1:15000 Goat anti-rabbit IRDye in 1:3 odyssey block:1X TBST (0.1% Tween) and 0.2% SDS for one hour at room temperature in the dark
N/A
Licor Odyssey
This antibody displays a consistent band at 125kDa. The only inconsistent thing about it is that in some samples, it shows up as a doublet while in others there is only a single band.
None
Shows up as a strong band near 125kDa
Some non-specific background but it's pretty weak.
This antibody works with little background and no optimization and is one of the cheaper that we have come across.