Abcam
Anti-PERK (phospho T980)
ab156919
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Browse Similar Products: PERK Western Blot Antibodies
PERK is a key protein that is phosphorylated and activated during ER stress, responsible for the phosphorylation eIF2alpha. Determining the activation state of PERK due to a variety of stress conditions is helpful in assessing the severity of ER stress.
Western Blot
H4IIE rat hepatoma liver cells
1:900 in 1:3 odyssey block:1X TBST (0.1% Tween) shaking, overnight at 4 degrees C
1:3 odyssey block:1X TBS at room temperature for 1 hr.
1:15000 Goat anti-rabbit IRDye in 1:3 odyssey block:1X TBST (0.1% Tween) and 0.2% SDS for one hour at room temperature in the dark
N/A
Licor Odyssey
There is a distinct band that shows up at 125kDa. The rest of the blot is relatively messy. Lots of non-specific binding
None
Antibody works and has a strong signal with minimal optimization
Lots of non-specific bands
Order Abcam's phospho-PERK if no other antibody has worked. Otherwise go with one that has a cleaner blot.