CD45 Antibody from BD Biosciences Review

Cell and Molecular Biology
Northwestern University
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

CD45-BV510 antibody

Catalog Number:

563530

The overall aim of my research is to investigate what target cells are available to SIV/HIV and in what abundance across various tissues. We used CD45 antibody to first get all leukocytes from the total cell pool.This product was selected because it works for Rhesus Macaque.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Rhesus Macaque PBMCs

Primary Incubation

1 hour (1:5 dilution)

Blocking Agent

Staining buffer with FBS from BD

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

The staining was very bright and it was easy to detect the leukocyte population.

Additional Notes

While using the isotype control was not necessary, we still decided to add it.

Related Categories

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Summary

The Good

Ease of use, good signal

The Bad

Price

The Bottom Line

Highly recommended clone for work in Rhesus Macaques.

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