Western Blot Application Review

Higuchi Bioscience Center
The University of Kansas
postdoc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Novus

Product Name:

GAP-43 Antibody

Catalog Number:

NBP1-92713

NBP1-92713 was used to detect the GAP-43 protein in mouse cortex samples by western blot. A single and clear band between 37kd and 50kd was detected with a standard western blot procedure.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Cortex

Primary Incubation

1:2000 in PBST, 4 degree overnight

Blocking Agent

2% milk

Secondary Incubation

1:5000 anti-mouse IgG, rt 2 hours

Tertiary Incubation

N/A

Detection

ECL

Results Summary

A clear and single band between 37kd and 50 kd was detected.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Specific and available with reactivity to rat, mouse, and human

The Bad

N/A

The Bottom Line

This product is good for the detecting GAP-3 by western blot.

Share your experience with other scientists. Write a Review! »

Join the discussion