Great Phospho-Src (Tyr527) Antibody

August 19, 2016

Pathology
University of Kansas Medical Center
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling Technology

Product Name:

Phospho-Src (Tyr527) Antibody

Catalog Number:

2105S

Western blot assays to validate protein array data in ovarian cancer cell lines.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Ovarian cancer cell line panel lysates

Primary Incubation

1:1000 in 5% BSA in TBS-T, overnight at 4 degrees C, on a rocker

Blocking Agent

5% BSA in TBS-T

Secondary Incubation

1:5000, anti-Rb in 5% BSA in TBS-T, 30 minutes at room temp, on a rocker

Tertiary Incubation

None

Detection

HRP Chemiluminescence

Results Summary

Very clean blot, only one clear band which is quite impressive for a phospho antibody.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Excellent phospho antibody, only one band

The Bad

None

The Bottom Line

Great antibody, available in smaller quantities to save money. Highly recommend.

Share your experience with other scientists. Write a Review! »

Join the discussion