Novus Biologicals
Syntaxin 5 Antibody
NBP1-80771
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Syntaxin-5 is known to locaize to the Endoplasmic reticulum-Golgi intermediate compartment membrane and Golgi apparatus (abundant in the cis-most cisternae) membrane. So its localization can be used as a marker of Golgi apparatus.
Immunofluorescence
HeLa cells
Overnight
5% BSA prepared in PBS
Coverslips were incubated for 1 hour at room temperature (in a dark chamber) with goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate 1:400 diluted in 1% BSA- PBS.
None
Coverslips were inverted onto a glass slide with a drop of mounting media and Imaged using Zeiss LSM 700 confocal fluorescence microscope.
The syntaxin 5 is stained in green and it does not seem to co-localize even partially with GM130 and syntaxin 5 staining seems to be non-specific.
syntaxin 5 staining seems to be non-specific.
Not good for immuno fluorescence as claimed by the manufacturer using themanufacturer suggested protocol.