Promega
CellTiter-Glo® Luminescent Cell Viability Assay
G7571
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CellTiter-Glo® Luminescent Cell Viability Assay was used to assay viability of cells treated with compounds that induced non apoptotic cell death in cancer cells. A pilot study was first conducted to ensure sensitivity of assay kit to detect increasing ATP levels increasing cell density
Viability Assay
Human U251 glioma cells
Human U251 glioma cells were seeded in 96 well (white opaque) tissue culture plates at different densities (see image). Viability was assayed after 24 hours of growth following the protocol provided by manufacturer. Protocol is easy. Combine equal proportions of substrate and buffer provided, apply on cells, mix and incubate for 10 minutes or more and read luminescence using a Luminometer.
This is a very easy assay to perform and protocol is easy to follow.
Result shows increasing luminescence readout with increasing cell density.
None
Assay works well and very easy.
Go for this assay to measure viability of cells.
Product Name: RealTime-Glo™ MT Cell Viability AssaySupplier Page