Abcam
Anti-LDL Receptor antibody [EP1553Y]
ab52818
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Browse Similar Products: LDL Receptor Western Blot Antibodies
Used this antibody for a western blot to determine LDL Receptor protein levels in U251 glioma cells. Cells were serum starved overnight, incubated with cycloheximide (10ug/ml) to block synthesis of new protein and then stimulated with 20% fetal bovine serum (FBS) for 4 hours or 8 hours. Parallel dishes of cells harvested at the start of stimulation with FBS were labeled as the time zero sample. LDL Receptor protein is diminished via degraded in lysosomal compartments when serum starved cells are exposed to serum.
Western Blot
U251 Glioma cell lysate
1:1000
5% milk in TBS 0.2%-Tween 20
1: 3000 with Goat-anti Rabbit antibody
None
Femto HRP Chemiluminescent substrate Kit
This antibody works well and detects specific band for LDL Receptor. LDL Receptor is decreased with increasing time course stimulation with fetal bovine serum
Specific band detected
Need to use a super signal ECL reagent
This antibody works well. Although it is costly, you get good results and there are not many commercially available LDL Receptor antibodies.