CD4 Antibody From BD Biosciences Review

August 17, 2016

Cell and Molecular Biology
Northwestern University
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Alexa Fluor® 700 Mouse Anti-Human CD4 Clone L200 (RUO)

Catalog Number:

560836

The purpose of the research is to study the resident HIV/SIV target cells in various Rhesus Macaque tissues.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Rhesus Macaque PBMCs

Primary Incubation

30 minutes on ice protected from ice

Blocking Agent

Staining buffer with FBS

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

Clear CD4 positive pool was visible

Additional Notes

None

Related Categories

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Summary

The Good

Ease of use, good signal

The Bad

Price

The Bottom Line

Good antibody to detect and separate CD4 positive cells from leukocyte population.

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