Great CD8a Antibody for Flow Cytometry

August 19, 2016

Microbiology
Medical College of Wisconsin
Research Technologist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

CD8 APC Antibody

Catalog Number:

100712

Mice are infected with virus. Splenocytes are collected, RBC lysed, Fc blocked, fixed, and stained for flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

1:100, 20 minutes

Blocking Agent

Fc Block

Secondary Incubation

1:500, 30 minutes, ice

Tertiary Incubation

NA

Detection

LSRII

Results Summary

Great signal for CD8 positive cell populations in our experiment.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Robust Amplifcation

The Bad

NA

The Bottom Line

Recommend this antibody

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