Great CD8a Flow Cytometry Antibody

August 18, 2016

Microbiology
Medical College of Wisconsin
Research Technologist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Biolegend

Product Name:

CD8 Pacific Blue Antibody

Catalog Number:

100728

Mice are infected with virus. Splenocytes are prepared using RBC lysis, Fc block, and fixing before flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

1:100, 20 minutes

Blocking Agent

Fc Block

Secondary Incubation

1:400, 30 minutes, ice

Tertiary Incubation

NA

Detection

NA

Results Summary

Antibody showed robust amplification with the cell samples we were using. Optimization was easy, and could probably be diluted down to a smaller concentration than what we used.

Additional Notes

None

Related Categories

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Summary

The Good

Robust Amplification

The Bad

NA

The Bottom Line

Would highly recommend this antibody for CD8 staining panels.

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