Biolegend
Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (FC) Antibody
400130
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Browse Similar Products: Mouse IgG1 Flow Cytometry Antibodies
The product was used to control for non-specific/Fc receptor binding of this isotype fluorophore combination in human synovial cells. It was chosen as it works in a pre-existing panel. Overall the antibody performed well with little non-specific binding detected.
Flow Cytometry
Synovial cells yielded by enzymatic digest
30 minute incubation at 4oC. Live/dead stain.
No specific blocking step. 2% FCS in staining buffer.
30 minute incubation at 4oC. Antibody cocktail used at this point containing antibody at 5ug/ml.
None
Mo Flow Astrios EQ 561 excitation 672/30 bandpass filter for emission
Antibody worked well to control for non-specific binding with little detected in an often difficult sample.
Antibody performed as expected allowing quantification of non-specific binding
No particular bad points
A decent antibody that works well at the recommended starting concentration