Biolegend
Brilliant Violet 510™ anti-human CD34 Antibody
343527
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Browse Similar Products: CD34 Flow Cytometry Antibodies
The product was used to stain cells yielded from human synovial tissue digested to phenotype the CD34 positive cell population. This product was chosen as it works well in a pre-existing flow cytometry panel. Overall antibody worked well giving a clear positive peak.
Flow Cytometry
Synovial cells yielded by enzymatic digest
30 minute incubation at 4oC. Live/dead stain.
No specific blocking step. 2% FCS in staining buffer.
30 minute incubation at 4oC. Antibody cocktail used at this point containing antibody at 5ug/ml.
None
Mo Flow Astrios EQ 488 excitation 530/40 bandpass filter for emission
Antibody worked well giving a clear CD34 positive population when used at the recommended starting dilution.
Antibody worked well and produced a clear positive population
No particular negative
A decent antibody on one of the new brilliant violet fluorophores that works well on what can be difficult samples