Very Useful Anti-Mouse CD4 Antibody

Biomedical Sciences
TTUHSC
Lead Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

FITC Rat Anti-Mouse CD4

Catalog Number:

553047

The aim of this experiment was to study the percentage of T cell subsets in mouse spleen. Single cell suspensions isolated from mouse splenocytes were stained with anti-mouse CD4 and CD8 antibody and analyzed by flow cytometry. The result demonstrates the presence of both CD4 and CD8 populations, which confirm the specificity of these antibodies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

30

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry in FITC Channel

Results Summary

The result show a clear CD4 and CD8 positive populations which confirm the specificity of the antibody

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Reproducibility

The Bad

None

The Bottom Line

Excellent FITC conjugated anti-mouse CD4 antibody

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