Very Good Anti-Mouse CD8 Antibody

August 12, 2016

Biomedical Sciences
TTUHSC
Lead Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

PE Rat Anti-Mouse CD8b

Catalog Number:

550798

We used this antibody to determine CD8 expression in mouse splenocytes. Single cell suspensions isolated from mouse splenocytes were used for staining with this anti-mouse CD8 PE antibody and the presence of CD8+ and CD8- population confirms the specificity of this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

30

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry in PE Channel

Results Summary

The results show a clear CD8 positive and negative populations which confirm the specificity of this antibody.

Additional Notes

None

Related Categories

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Summary

The Good

Reproducibility

The Bad

None

The Bottom Line

Very good anti-mouse CD8 PE antibody

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