Biolegend Anti-Mouse B220 Antibody For Flow Cytometry

August 11, 2016

Microbiology and Immunology
Virginia Commonwealth University
MD/PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

APC anti-mouse/human CD45R/B220 Antibody

Catalog Number:

103212

This antibody was used for the identification of B-cells from murine spleens and lymph nodes. We selected this product because of its cost and ease of use.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

30 minutes at 4C

Blocking Agent

Anti-mouse CD16/32

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fortessa Flow Cytometer

Results Summary

B-cells from mouse spleens were consistently and reliably identified using this antibody. Very little background staining observed.

Additional Notes

Also works for fluorescent immunohistochemistry

Related Categories

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Summary

The Good

Cost and ease of use

The Bad

None

The Bottom Line

Would highly recommend this product. We have not experienced any issues with it yet.

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