SC PRDM6 Antibody Review

Genetics
Yale
Tech

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

PRDM6 (M-159) Antibody

Catalog Number:

sc-98736

Our lab generates a lot of protein knockouts in mouse embryonic stem cells using crispr-cas9 technology. We use Western Blots and subsequent sequencing to screen potential knockout clones.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse ES cells

Primary Incubation

1:1000 4C overnight

Blocking Agent

5% milk

Secondary Incubation

1:3000 in 5% milk for one hour at RT

Tertiary Incubation

N/A

Detection

Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad

Results Summary

Antibody doesn't work. No bands detected, even after repeating the western blot. Might be a faulty batch.

Additional Notes

None

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Summary

The Good

Santa Cruz does easy refunds

The Bad

Bad batch--no bands detected

The Bottom Line

Might not be ideal for blots on mouse ES cells.

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