c-Cbl Antibody CST Review

Genetics
Yale
Tech

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

c-Cbl Antibody

Catalog Number:

2747S

Our lab generates a lot of protein knockouts in mouse embryonic stem cells using crispr-cas9 technology. We use Western Blots and subsequent sequencing to screen potential knockout clones.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse ES cells

Primary Incubation

1:1000 4C overnight

Blocking Agent

5% milk

Secondary Incubation

1:3000 in 5% milk for one hour at RT

Tertiary Incubation

N/A

Detection

Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad

Results Summary

Great antibody, clear signal, minimal background, would use again.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Clear band, strong antibody

The Bad

Expensive

The Bottom Line

Great antibody, would use again.

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