Santa Cruz Biotechnology
PARP-1 Antibody (H-250)
sc-7150
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Browse Similar Products: PARP-1 Western Blot Antibodies
Poly(ADP-ribose) polymerase-1 (PARP-1), also designated PARP, is a stress protein that is also a marker for apoptosis. PARP also has the ability to induce programmed cell death, via the production of PAR, which stimulates mitochondria to release AIF. We used this molecule to detect apoptosis upon treatment.
Western Blot
Whole cell lysates treated with different toxic agents
Diluted 1:500 in blocking solution, incubated for overnight at 4 degrees
5% non fat dry milk in TBST
Anti-rabbit secondary antibody
None
ECl
This antibody gives 3 non specific bands sometimes so stringent blocking is required.
Reproducibility of results
Nothing
Overall, its a good antibody