Great Antibody To Detect His Tagged Proteins

Department of Microbiology and Immunology
University of Illinois, Chicago
PostDoctoral Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-6X His tag® antibody (HRP)

Catalog Number:

ab1187

His tag is commonly used for purification of protein based Ni NTA chromatography. We used this antibody to detect the purification of proteins.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Purified recombinant protein elutes

Primary Incubation

Diluted 1: 1000 in blocking solution, incubated for overnight at 4 degrees

Blocking Agent

5% non fat dry milk in TBST

Secondary Incubation

None

Tertiary Incubation

None

Detection

ECl

Results Summary

His tagged proteins can be detected by this antibody

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

No non-specific reactivity

The Bad

Nothing

The Bottom Line

Its a nice HRP conjugated antibody. It saves time and effort as well.

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