Cell Signaling
DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody)
2368
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Molecular biology research involves identification, characterization and functional analysis of DNA, RNA and proteins. To understand the cellular processes at the molecular level, recombinant protein production is often desired. To purify and detect the recombinant proteins, certain tags such as myc, flag and his6 are used. Flag tag is often used for immunoprecipitation related experiments. We used Flag tag antibody to detect the recombinant protein levels post purification.
Western Blot
Purified recombinant protein elutes
Diluted 1: 500 in blocking solution, incubated for overnight at 4 degrees
5% non BSA in TBST
Anti-rabbit secondary antibody
None
ECl
Flag tag can be detected by this antibody but the bands were diffused.
Reproducible results
It does not give crisp bands
Overall, a decent antibody