Nice Western Blot Antibody For EGFR

Department of Microbiology and Immunology
University of Illinois, Chicago
PostDoctoral Research Fellow

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Company:

Santa Cruz Biotechnology

Product Name:

EGFR (1005)-G Antibody

Catalog Number:

sc-03-G

Browse Similar Products: EGFR Western Blot Antibodies

Epithelial growth proliferation factor (EGFR) is present on cells that is involved in cellular proliferation. Upon ligand binding, it undergoes dimerization and autophosphorylation which further mediates the signals to downstream modules. It is often overexpressed in several cancers and is targeted for anti-cancer therapeutics. We used anti-EGFR antibody to determine differential EGFR levels in several cancer cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Whole cell lysates of different cancer cells

Primary Incubation

Diluted 1: 1000 in blocking solution, incubated for overnight at 4 degrees</p

Blocking Agent

5% non fat dry milk in TBST

Secondary Incubation

Anti-goat secondary antibody

Tertiary Incubation

None

Detection

ECl

Results Summary

We could determine the EGFR expression in different human breast cancer cells.

Additional Notes

None

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Summary

The Good

Reproducible results

The Bad

Nothing

The Bottom Line

It's a nice western blotting antibody in cancer research

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