A Decent Antibody For CDK6 Analysis

Department of Microbiology and Immunology
University of Illinois, Chicago
PostDoctoral Research Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell signaling

Product Name:

CDK6 (D4S8S) Rabbit mAb

Catalog Number:

13331

Cyclin dependent kinases play a vital role in driving cell cycle progression in an efficient and errorproof manner. CDK6 activity is observed during progression through the G1/S-phase of the cell cycle. We used the CDK6 antibody to determine the cell cycle arrest in cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Whole cell lysates treated with different toxic agents

Primary Incubation

Diluted 1: 1000 in blocking solution, incubated for overnight at 4 degrees

Blocking Agent

5% non fat dry milk in TBST

Secondary Incubation

Anti-rabbit secondary antibody

Tertiary Incubation

None

Detection

ECl

Results Summary

It's a good antibody and can detect the over-expression of CDK6.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Reproducible results

The Bad

Nothing

The Bottom Line

It's a nice antibody for routine western blotting

Share your experience with other scientists. Write a Review! »

Join the discussion