A Nice Antibody For Total AKt

Department of Microbiology and Immunology
University of Illinois, Chicago
PostDoctoral Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-pan-AKT antibody

Catalog Number:

ab8805

Akt is a key regulator in several cellular functions. It is phosphorylated and mediates the signals to nucleus of cell. Total AKT is often used as a control to determine relative phosphorylation of AKt.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Whole cell lysates treated with different toxic agents

Primary Incubation

diluted 1: 500 in blocking solution, incubated for overnight at 4 degrees

Blocking Agent

5% non fat dry milk in TBST

Secondary Incubation

Anti-rabbit secondary antibody

Tertiary Incubation

None

Detection

ECl

Results Summary

This antibody works every time and gives total AKT band specifically

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Single band with no non-specific reactivity

The Bad

Nothing

The Bottom Line

It's a good antibody for western blotting

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