Santa Cruz Biotechnology
GAPDH Antibody (G-9)
sc-365062
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GAPDH plays a crucial role in the process of glycolysis. Because of its vital role in glucose metabolism in cell, it is often used as a control to ensure equal loading of samples in western blotting and RT-qPCR. We used this as a internal loading control for western blotting.
Western Blot
Whole cell lysates of different cancer cells
Diluted 1: 1000 in blocking solution, incubated for overnight at 4 degrees
5% non fat dry milk in TBST
Anti-mouse secondary antibody
None
ECl
We confirmed equal loading in all samples by using GAPDH antibody.
Single band with no non-specific reactivity
Nothing
A good antibody