EMD Millipore
Anti-Matrix Metalloproteinase 2
04-1048
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Browse Similar Products: MMP-2 Western Blot Antibodies
Western blot of HEK293 cells over expressing human MMP2 cDNA.
Western Blot
HEK293 transiently transfected with human MMP2 cDNA. Lane 1: Intracellular protein lysate 24 hours post-transfection. Lane 2: Extracellular secretred MMP2 collected from cultured media 24 hours post-transfection
1:1000 in 2% milk incubated at 4C overnight
5% milk
Anti-Rabbit-HRP (1:3000) in 2% milk incubated for 1 hour at room temperature
N/A
ECL Western Blotting substrate (Promega #W1001)
Intracellular MMP2 appears as two bands- unfolded and folded expressed protein.
Antibody worked well to detect overexpressed human MMP2. Gave very specific signal.
None to report
I would recommend this antibody to investigators.