Good Anti-Mouse CD3 Antibody

Biomedical Sciences
TTUHSC
Lead Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PerCP/Cy5.5 anti-mouse CD3 Antibody

Catalog Number:

100217

We used this antibody to determine the percentage of CD3 positive T cells in the spleen of Balb/C mouse. Single cell suspensions were isolated from Balb/C mouse splenocytes and were stained with PerCP/Cy5.5 conjugated anti-mouse CD3 antibody. The result shows a clear CD3+ and CD3- populations which confirms the specificity of this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

45 min

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometric detection in PerCP/Cy5.5 channel.

Results Summary

The results show a clear CD3 positive and negative population which confirm the specificity of the antibody

Additional Notes

None

Related Categories

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Summary

The Good

Great specificity

The Bad

None

The Bottom Line

Good anti-mouse CD3 PerCP/CY 5.5 antibody

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