This Anti-TRPV1 Antibody Can Detect Real Signal

Anesthesiology
University of Wisconsin
Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Neuromics

Product Name:

anti-TRPV1 antibody

Catalog Number:

#RA10110

Aim of research was to detect subset of TRPV1 positive neurons and to detect whether some of those are also IB4 positive. This TRPV1 antibody was selected because company promised that antibody will be TRPV1 specific. Result shows that some of small diameter neurons are positive for TRPV1 and also negative for IB4 (lectin).

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Rat dorsal root ganglion

Primary Incubation

Overnight

Blocking Agent

Normal Donkey Serum 10%

Secondary Incubation

2 hours

Tertiary Incubation

None

Detection

Leica confocal microscope

Results Summary

Real staining is detected, however some background is noticed

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Real staining is detected

The Bad

Some background is present

The Bottom Line

This antibody can be efficiently used for IHC, however some background is also present.

Share your experience with other scientists. Write a Review! »

Join the discussion