This Is An Excellent Anti-Synaptophysin Antibody

Anesthesiology
University of Wisconsin
Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

EMD Millipore

Product Name:

anti-Synaptophysin

Catalog Number:

MAB329


Aim of research was to detect intensity of synaptophysin in IB4-positive terminals. Result shows that there is some colocalization, however no significant increase of synaptophysin in IB4-positive terminals was observed in comparison to IB4 negative terminals.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse spinal cord

Primary Incubation

Overnight

Blocking Agent

Normal Donkey Serum 10%

Secondary Incubation

2 hours

Tertiary Incubation

None

Detection

Leica confocal microscope

Results Summary

Clear synaptic labeling is detected in all synapses, including IB4 positive terminals.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Strong synaptic staining

The Bad

None

The Bottom Line

This is good antibody

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