#AF909 Is Good Antibody

Anesthesiology
University of Wisconsin
Scientist

Overall

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Company:

R&D Systems

Product Name:

anti-MMP9 antibody

Catalog Number:

AF909

Aim of this research was search for specific anti-MMP-9 antibody. Company recommended this antibody to be specific. To validate it I transfected PC12 cells with MMP9 and confirmed that transfected cells has much brighter signal detected by antibody in comparison to non-transfected cells.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

PC-12 cells

Primary Incubation

2 hours

Blocking Agent

Normal Donkey Serum 10%

Secondary Incubation

1 hour

Tertiary Incubation

None

Detection

Leica confocal microscope

Results Summary

Antibody detect a real signal. This is good antibody.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Real staining

The Bad

None

The Bottom Line

Real staining

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