Biolegend
PE/Cy7 anti-human CD194 (CCR4) Antibody
359409
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This antibody was used for a phenotypic analysis of human PBMCs. CCR4 was used to identify T-helper cell subsets in peripheral blood using flow cytometry. Healthy donors were compare to patients with arthritis. The staining was very reliable
Flow Cytometry
Human PBMCs
0.5 Mio. of human peripheral blood was stained with the CCR4 antibody. Cells were incubated for 30 minutes at room temperature. After incubation, cells were washed with 1ml PBS for three times and cells were resuspended in 300ul FACS buffer for direct usage for flow cytometry.
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This antibody provides a clear separation of CCR4+ and CCR4- human PBMCs
Good staining of CCR4 human PBMCS
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This is a good antibody to use for phenotypic analysis of human PBMCs