Good Antibody For Western Bloting

July 28, 2016

Department of Microbiology and Immunology
University of Illinois, Chicago
PostDoctoral Research Fellow

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Company:

Cell Signaling Technology

Product Name:

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody

Catalog Number:

9101

pERK is MAPK involved in cellular proliferation and apoptosis. It's upregulation is associated with cancer growth and progression.

Experimental Design and Results Summary

Applications

Western Blot

Sample

293 T cell lysate

Primary Incubation

1: 1000 in blocking solution for overnight at 4 degrees

Blocking Agent

5% non fat dry milk in TBST

Secondary Incubation

Anti rabbit HRP for an hour

Tertiary Incubation

None

Detection

ECL

Results Summary

Upon stimulation with EGF, there is induction of pERK in 293T cells.

Additional Notes

None

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Summary

The Good

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The Bad

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The Bottom Line

Good antibody for signaling studies

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