Great Loading Control Antibody

Department of Microbiology and Immunology
University of Illinois, Chicago
PostDoctoral Research Fellow

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Company:

Cell Signaling Technology

Product Name:

β-Actin (13E5) Rabbit mAb

Catalog Number:

4970

Beta actin is a conserved cytoskeletal protein which is present in all cells. This is often used in RNA and protein based experiments as a loading control. We routinely use this molecule as an internal control for our experiments. We have tried several antibodies for beta actin and cell signaling technology makes one of the best antibodies for beta actin.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Different cancer cells

Primary Incubation

1: 1000 in blocking solution for overnight at 4 degrees

Blocking Agent

5% non fat dry milk in TBST

Secondary Incubation

Anti rabbit HRP for an hour

Tertiary Incubation

None

Detection

ECL

Results Summary

Single specific band was observed for beta actin.

Additional Notes

None

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Summary

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The Bad

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The Bottom Line

Amazing antibody

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