Cdk Antibody With High Unspecificity

Protein Degradation
WGL
PI

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology Inc.

Product Name:

Cdc2 (aP-17)

Catalog Number:

sc-12826

Target: Arabidopsis CDKA;1. Did not work with two different goat anti-rabbit secondary antibodies in various concentrations from Thermo or Santa Cruz using pico or femto detection (ECL kit;Thermo) or ECL detection (Millipore)

Experimental Design and Results Summary

Applications

Western Blot

Sample

Arabidopsis seedlings or leaves

Primary Incubation

1 to 1000, overnight

Blocking Agent

5% milk TBST

Secondary Incubation

1 to 2500

Tertiary Incubation

n.a.

Detection

ECL

Results Summary

Did not work with secondary antibody from Thermo using pico or femto detection (ECL kit;Thermo)

Additional Notes

We recommend sc-53

Related Categories

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Summary

The Good

n.a.

The Bad

Doesn't work, apparently never tested

The Bottom Line

Useless, did not get a refund

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