Anti-MBP Purification Tag Antibody

July 29, 2016

Protein Degradation
WGL
PI

Overall

Quality of Results

Ease-of-Optimization

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Company:

New England Biolabs

Product Name:

Polyclonal anti-MBP antibody from rabbit

Catalog Number:

E8032S

MBP affinity purification tag can be easily detected using this antibody and therefore used as loading or interaction control in various experimetns such as heterologous protein expresssion and purification or pull-down studies.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Recombinant protein from Escherichia coli extracts

Primary Incubation

1 to 1000, overnight

Blocking Agent

5% milk TBST

Secondary Incubation

1 to 2500

Tertiary Incubation

n.a.

Detection

ECL

Results Summary

MBP affinity purification tag can be easily detected using this antibody and therefore used as loading or interaction control in various experimetns such as heterologous protein expresssion and purification or pull-down studies.

Additional Notes

None

Image Gallery

Summary

The Good

Very good clear signal and reproducibility

The Bad

n.a.

The Bottom Line

Very good clear signal and reproducibility

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