Highly Specific Antibody For Hygromycin Resistance

Protein Degradation
WGL
PI

Overall

Quality of Results

Ease-of-Optimization

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Company:

St John's Laboratory Ltd

Product Name:

HPT

Catalog Number:

STJ10181

Browse Similar Products: HPT Western Blot Antibodies

We analyze samples of crude Arabidopsis thaliana (mouse-ear cress) protein extract from seedlings or later developmental stages and bacterial extracts according to their protein content. We use this specific antibody to determine whether the target is expressed in the plant by standard western blotting. This antibody is one of the few reagents working for detection, we tested several comparable products from various companies.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Arabidopsis seedlings or leaves

Primary Incubation

1 to 1000, overnight

Blocking Agent

5% milk TBST

Secondary Incubation

1 to 2500

Tertiary Incubation

n.a.

Detection

ECL

Results Summary

This antibody works excellently for the detection of hygromycin resistance protein HPT. HPT can be easily detected using this antibody.

Additional Notes

none

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Summary

The Good

Very good clear signal and reproducibility. Only commercially available antibody that worked in our hands.

The Bad

None

The Bottom Line

Highly specific antibody for hygromycin resistance

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