Staining PBMC With Anti-CD3 BV605

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-human CD3 BV605

Catalog Number:

317321

This antibody was used as a T cell marker in a T cell phenotyping panel for flow cytometry. It was chosen because it is a commonly used clone, BV605 is bright enough to fully resolve positive cells from negative, and Biolegend had the best price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

5uL per test in 100uL total staining volume for 20 minutes at room temperature

Blocking Agent

2% FBS

Secondary Incubation

n/a

Tertiary Incubation

n/a

Detection

n/a

Results Summary

CD3 staining was as expected, with positive cells clearly resolved from negative cells.

Additional Notes

None

Related Categories

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Summary

The Good

Works well. Low cost

The Bad

n/a

The Bottom Line

I will definitely use this antibody again.

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