Staining Mouse WBC With Anti-CD3 APC

August 10, 2016

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Anti-mouse CD3 APC

Catalog Number:

560590

This antibody was used to stain T cells in mouse peripheral blood. It was chosen because it is a commonly used clone and APC is a bright fluorochrome.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

ACK-lysed peripheral mouse blood

Primary Incubation

1uL per test in 100uL total staining volume for 20 minutes at room temperature

Blocking Agent

1:50 dilution of anti-mouse FcBlock and 2% FBS.

Secondary Incubation

n/a

Tertiary Incubation

n/a

Detection

n/a

Results Summary

CD3 staining was as expected, and did not spill over into the PerCP/Cy5.5 channel.

Additional Notes

None

Related Categories

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Summary

The Good

Works well. Low cost

The Bad

n/a

The Bottom Line

I will definitely continue to use this antibody.

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