Staining Mouse RBC With Anti-TER-119 FITC

August 02, 2016

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-mouse TER-119 FITC

Catalog Number:

116206

This antibody was used as a RBC marker in a hematopoietic lineage panel. It was chosen because it is a commonly used clone, spillover from FITC staining to other markers will be minimal since TER-119 is specific to RBC, and Biolegend had the best price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

peripheral mouse blood

Primary Incubation

1uL per test in 100uL total staining volume for 20 minutes at room temperature

Blocking Agent

1:50 dilution of anti-mouse FcBlock and 2% FBS.

Secondary Incubation

n/a

Tertiary Incubation

n/a

Detection

n/a

Results Summary

TER-119 staining was as expected, with 100% of RBC exhibiting a strong signal in the FITC channel compared to isotype control.

Additional Notes

None

Related Categories

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Summary

The Good

Works well. Low cost

The Bad

n/a

The Bottom Line

I will continue to use this antibody in this panel.

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