Staining Mouse WBC With Anti-F4/80 APC

July 29, 2016

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Biolegend

Product Name:

Anti-mouse F4/80 APC

Catalog Number:

123116

This antibody was used to stain macrophages in mouse peripheral blood. It was chosen because it is a commonly used clone, a bright fluorochrome, and Biolegend had the best price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

ACK-lysed peripheral mouse blood

Primary Incubation

1uL per test in 100uL total staining volume for 20 minutes at room temperature

Blocking Agent

1:50 dilution of anti-mouse FcBlock and 2% FBS.

Secondary Incubation

n/a

Tertiary Incubation

n/a

Detection

n/a

Results Summary

Macrophage staining was low, but as expected, in peripheral blood.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Works well. Low cost

The Bad

n/a

The Bottom Line

Will use again.

Share your experience with other scientists. Write a Review! »

Join the discussion