Biolegend
Anti-mouse CD3 Pacific Blue
100213
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Browse Similar Products: CD3 Flow Cytometry Antibodies
This antibody was used as a T cell marker in a hematopoietic lineage panel. It was chosen because it is a commonly used clone, Pacific Blue is bright enough to fully resolve positive cells, and Biolegend had the best price.
Flow Cytometry
ACK-lysed peripheral mouse blood
1uL per test in 100uL total staining volume for 20 minutes at room temperature
1:50 dilution of anti-mouse FcBlock and 2% FBS.
n/a
CD3 staining was as expected.
None
Works well. Low cost
I will definitely continue to use this antibody in the panel.