Biolegend
Anti-mouse TCR-beta Pacific Blue
109225
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This antibody was used to phenotype TCR-beta expressing T cells. It was chosen because it is specific, bright enough to fully resolve positive cells, and Biolegend had the best price.
Flow Cytometry
ACK-lysed peripheral mouse blood
1uL per test in 100uL total staining volume for 20 minutes at room temperature
1:50 dilution of anti-mouse FcBlock and 2% FBS.
n/a
Staining of TCR-beta was as expected.
None
Works well. Low cost
I will definitely use this again.