Staining PBMC With Anti-CD4 PE/Cy7

July 22, 2016

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

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Quality of Results

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Company:

Biolegend

Product Name:

Anti-human CD4 PE/Cy7

Catalog Number:

300512

This antibody was used to phenotype CD4+ T cells so that cytokine production could be analyzed. It was chosen because it is a commonly used clone, PE-Cy7 is a bright fluorochrome, and Biolegend had the best price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

5uL per test in 100uL total staining volume for 20 minutes at room temperature

Blocking Agent

2% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

N/A

Results Summary

Staining of CD4+ T cells was as expected.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Bright, specific staining

The Bad

N/A

The Bottom Line

I will definitely use this again.

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