Cell Signaling Technology
Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb
9664-S
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We used this antibody to look at autophagy occurrence and inhibition in mouse ES cells after different treatments.
Western Blot
Mouse ES cells
1:1000 4C overnight
5% milk
1:3000 in 5% milk for one hour at RT
N/A
Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad
We saw a clear band at the expected size. Better than another antibody we tried previously.
None
Works well
Expensive
Great antibody!