Centrifugation Of RNA And Protein Samples At 4 Degree Celsius

July 15, 2016

University of Birmingham
Biosciences
Research Assistant

Overall

Performance

Ease-of-Optimization

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Company:

Eppendorf

Product Name:

Centrifuge 5424R

Catalog Number:

5424R

We use Eppendorf's bench-top microcentrifuge (Refrigerated model) for prepping samples for RNA extraction and protein samples routinely in our laboratory. This centrifuge has maximum speed up to 21000 G and with temperature range starting from -10 degree C to 40 degree C. It also has fast cooling program which takes only 8 minutes to cool the unit to 4 degree C. The centrifugation using this machine for our samples were satisfactory and never had any issue.

Experimental Design and Results Summary

Application

Centrifugation of RNA and protein samples (less than 2 ml volume)

Starting Material

Bacterial cell culture for prepping RNA and protein samples

Protocol Overview

Less than 2 ml of bacterial culture samples to be used for RNA or protein extraction were concentrated/pelleted in 2 ml sterile eppendorf tubes at a speed of 15000 rpm at 4 degree C for 10 minutes. Rest of the centrifugation steps are followed as per the kit's specifications.

Tips

Pre-cool centrifuge to 4 degree C using fast temperature program before starting actual centrifugation of the samples

Results Summary

Centrifugation performs very nice with less vibration and sound. It has 24 place 1.5/2.0 ml rotor. Broad range of temperature program and can go up to 21000 G speed. Centrifugation programming is very simple and straight forward to use. Always, we had no issues while performing centrifugation using this centrifuge.

Features Summary

Ideal for RNA and protein samples prepping at 4 degree Celsius temperature

Additional Notes

None

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Summary

The Good

Installation of the centrifuge is easy. Programming/settings for centrifugation are very simple.

The Bad

None

The Bottom Line

Ideal centrifuge with refrigeration temperature for the samples which requires centrifugation at lower temperatures especially molecular biology laboratories.

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