Efficient Removal Genomic DNA Contamination From Total RNA Samples

Protocol Overview

First, I checked the quantity of total RNA present in my samples using Qubit based RNA quantification assay. All of my samples were less than 250 microgram/ml. Then I followed DNA digestion using Turbo DNase in a 50 microliter reaction volume as recommended by the kit's protocol. Used 1 microliter enzyme to digest genomic DNA. Remaining steps were followed exactly as mentioned in the kit's instructions. Finally Turbo DNase enzyme was removed and total RNA was transferred to fresh tube. Few aliquots of this DNase digested samples were used as template in a PCR reaction to check removal of the genomic DNA.